ULB, CHU Saint-Pierre, Hôpital Erasme, ULiège - FNRS

" The project investigates the immune responses to SARS-CoV-2 infection in humans. Cohorts of patients and contacts are recruited and followed-up during several months following infection. Using systems serology and systems biology approaches, the quality and dynamics of antibody and cellular responses are analysed to identify correlates of protection against severe disease and to determine the duration of natural immunity to the virus. The project should provide insight in the host response to SARS-CoV-2 and should help define deconfinement strategies and develop prophylactic and therapeutic interventions against COVID-19. "
Funding: COVID 19 - ULB
Contact: Arnaud Marchant: This email address is being protected from spambots. You need JavaScript enabled to view it. Stanislas Goriely: This email address is being protected from spambots. You need JavaScript enabled to view it.

ULiège - Royal Academy of Sciences, Letters and Fine Arts of Belgium
"The Centre for Protein Engineering (CIP, ULiège), through its two technological platforms, has been involved in the production of reagents (i.e. proteins) for diagnostic and analyses of SARSCoV-2 infection. In particular, Protein Factory has produced a pure and highly active enzyme (CAS12) for a CRISPR/CAS assay, which, in combination with classical PCR technology allows rapid detection of the virus. Automated screening is currently performed at Robotein for optimal formulation of CAS12. The production of complementary protein reagents for such tests is underway. Furthermore, in a collaborative effort with Dr Arnaud Marchant at Institut d'Immunologie Médicale (ULB), the CIP is involved in the design and production of recombinant SARS-COV-2 antigens towards the development of a platform for high dimensional systems serology analysis of SARS-CoV-2 antibodies."
Contact: André Matagne This email address is being protected from spambots. You need JavaScript enabled to view it. 

UNamur - Royal Academy of Medicine of Belgium - Royal Academy of Sciences, Letters and Fine Arts of Belgium
" UNamur researchers have developed a procedure for the detection of SARS-CoV-2. The protocol has been approved by the Federal Agency for Medicines and Health Products (FAMHP). As successfully realized within UNamur, the method can be implemented in a molecular biology research lab to be used for diagnostic purposes on clinical samples. It requires little technological development and allows to overcome reagents shortage or availability of automated systems. To support the implementation of the diagnostic procedure, the required equipment and logistical means have been described in a "starting pack" document accompanying the protocol. "
Publication References: The protocol and starting pack can be downloaded here: https://www.narilis.be/news/sana-sars-cov-2-namur
Contact: Virginie Bourguignon This email address is being protected from spambots. You need JavaScript enabled to view it.

ULB, Department of Pathology, Erasme hospital and DIAPath-CMMI - Royal Academy of Medicine of Belgium
" A first objective of this project is to establish an accurate topography of the SARS-CoV-2 virus in the organs and various cells of patients who died in COVID-19 units, in order to contribute to a better understanding of the pathogenesis of COVID-19. A second objective is to provide a routine diagnostic test for the detection of SARS-CoV-2 in routine samples in pathological anatomy. "
Funding: Fonds Y Boël (Brussels, Belgium), Fonds Erasme pour la Recherche Médicale (Brussels, Belgium), and "Appel à projet Spécial COVID-19 - ULB" (Brussels, Belgium). The CMMI is supported by the European Regional Development Fund and the Walloon Region of Belgium (Wallonia-biomed; grant no. 411132-957270; project "CMMI-ULB").
Contact: Prof. I. Salmon, Department of Pathology, Erasme Hospital, Université Libre de Bruxelles (ULB), Route de Lennik 808, 1070 Brussels, Belgium.

UCLouvain - Royal Academy of Sciences, Letters and Fine Arts of Belgium
" Optimisation of bacterial strains for the expression of proteins from the COVID-19 virus Optimisation of bacterial strains for the expression of proteins from the COVID-19 virus Optimisation of bacterial strains for the expression of proteins from the COVID-19 virus "
Funding: FNRS
Contact: This email address is being protected from spambots. You need JavaScript enabled to view it.

ULiège - FNRS - Royal Academy of Medicine of Belgium
" Massive infiltration of activated macrophages is associated with the development of a cytokine storm that leads to severe acute respiratory syndrome induced by SARS-CoV-2. Based on our results demonstrating the key role exerted by epigenetic regulation in the activity of macrophages, we propose to study the methyltransferase EZH2 in the pathogenesis induced by SARS-CoV-2. We have also shown that the PD-1 immune checkpoint is responsible for the macrophage anergy induced by the absence of EZH2. The goal of the project is to temper the excessive inflammatory responses associated with macrophages by interfering on the EZH2-PD-1 axis. "
Funding: Decision pending (FNRS Crédit Urgent de Recherche 40002642)
Publication References: - Inhibition of EZH2 methyltransferase decreases immunoediting of mesothelioma cells by autologous macrophages through a PD-1-dependent mechanism. Malik Hamaidia, Hélène Gazon, Clotilde Hoyos, Gabriela...

Read more: Luc Willems, FNRS Research Director

ULB - Royal Academy of Medicine of Belgium
" The project aims at understanding why men are more affected than women. Compelling evidences indicate sex-bias in SARS-CoV2 with male representing the majority of severe cases requiring intensive care. Excessive inflammatory symptoms attributed mainly to cytokine storm is the hall-mark of disease progress. We ask whether differences in genetic fitness between male and female patients could contribute to sex-bias and we focus, in particular, on potential role of microRNAs in fine tuning the type and magnitude of the inflammatory response "
Funding: Current application to FNRS IRIS research
Publication References: - Chamekh M, M Deny, M Romano, et al. Differential Susceptibility to Infectious Respiratory Diseases between Males and Females Linked to Sex-Specific Innate Immune Inflammatory Response. Front Immunol. 2017; 8: 1806. - Casimir, G. J., Lefèvre, N., Corazza, F et al. J. Sex and inflammation in respiratory diseases : a clinical...

Read more: Mostafa Chamekh, Professor (coordinator of the project) - Georges Casimir, Professor - Francis...

LHUB-ULB - Royal Academy of Medicine of Belgium
" "The purpose of this study is to map the healthcare workers exposure risk in Saint-Pierre University Hospital by combining molecular methods with serological assays. In summary, the short-term objective is threefold: (1) Provide an estimate of the sero-prevalence at baseline among high-risk health care workers (2) Determine prospectively the incidence of new SARS-CoV-2 infections among high-risk health care workers (3) Estimate the healthcare worker exposure to the virus by serological data to help our five partner hospitals in their day-to-day management of the ongoing pandemic The mid-term objectives are: (1) Establish an accurate mapping of the spread of the virus among our population allowing to identify the chain of transmission, (2) Identify predictive immunological markers of susceptibility to infection and disease severity to inform vaccine strategies and, (3) Establish solid diagnosis algorithm that will help adequate lockdown...

Read more: O. Vandenberg, Pr. - N. Clumeck, Pr. - C. Montesinos, Pr. - N. Dauby, MD, PhD - C. Gilles, MD - C....

ULiège, CHU - Royal Academy of Medicine of Belgium
" Nous faisons face à une pandémie d'infection COVID-19. Ce virus pathogène est source de complications pulmonaires aggravant fortement le pronostic de nos patients. De nos jours, tant les mécanismes physiopathologiques en cause que les marqueurs de sévérité de l'atteinte restent indéterminés. Il apparaît donc comme urgent de pouvoir identifier des marqueurs diagnostiques, pronostiques et prédictifs pour nos patients. Dans ce contexte, plusieurs voies rapidement explorables nous apparaissent comme critiques. Le but final de cette étude est de contribuer à une prise en charge plus ciblée des patients porteurs d'une infection au SRAS-Cov-2. "
Funding: Fonds Léon Frederic
Publication References: Dix publications représentatives : 1) Prevention of murine radiogenic thymic lymphomas by tumor necrosis factor or by marrow grafting. HUMBLET C, GREIMERS R, DELVENNE P, DEMAN J, BONIVER J, DEFRESNE MP. J Natl Cancer Inst 88 (1996), 824-831. IF...

Read more: Ph. DELVENNE, FNRS Research Director / Chef de service Anatomie pathologique - J. GUIOT, Chef de...

GSK - Royal Academy of Medicine of Belgium
" Support covid international: https://www.gsk.com/en-gb/media/resource-centre/our-contribution-to-the-fight-against-2019-ncov/ Support covid en Belgique: https://be.gsk.com/media/915368/cp-gsk-contributions-majeures-de-gsk-a-la-lutte-contre-le-covid-19.pdf NA NA "
Funding: NA
Contact: Philippe Denoel - This email address is being protected from spambots. You need JavaScript enabled to view it.

CMMI
" The Center of Microscopy and Molecular Imaging (CMMI) is currently involved in establishing an accurate SARS-CoV-2 virus distribution topography in the organs and various cells of patients who died in COVID-19 units by providing immunohistochemistry and electron microscopy services (study director Pr. Isabelle Salmon). We have also developed and are currently offering preclinical multimodal quantitative imaging - of lung inflammation - of bacterial population (pathogen or no pathogen) - and of viral infection by isotopic imaging. All these models enable the understanding of pulmonary pathological features caused by SARS-viruses such as CoVID-19. From molecular to humanized animal models, the CMMI provides customized imaging solutions to research projects on Coronavirus The CMMI is also a proud partner of the ULB diagnostic platform set-up in Gosselies to face the epidemic of SARS-CoV2. "
Contact: This email address is being protected from spambots. You need JavaScript enabled to view it.
Comment: The CMMI was co-founded by ULB et UMons and is supported...

Read more: Serge Goldman, Scientific Director

GIGA-Institute, ULiège - FNRS - Royal Academy of Sciences, Letters and Fine Arts of Belgium
" The main objective of our research program is the identification of human genetic factors impacting the COVID19 pandemic. Within the COVID-19 host genetics initiative (https://www.covid19hg.org/), we will establish a shared set of phenotypes to be combined with genomic data for standard GWAS and further meta-analysis. Field of expertise of our laboratory : genetics, genomics, bioinformatics, immune-response related diseases. In addition, the GIGA Institute is an interdisciplinary research institute from the ULiege devoted to academic excellence in the biomedical sciences to foster ground-breaking medical innovation. It comprises 583 members attached to six faculties (Medicine, Science, Veterinary Medicine, Engineering, Agbiotech, Psychology) that are organized in four disease-based (neuroscience, cancer, infection-inflammation-immunity, cardiovascular) and two method-based thematic units...

Read more: Souad Rahmouni, Doctor

ULiège - Royal Academy of Medicine of Belgium
" In the context of the COVID-19 pandemic, this project involves collecting samples of human bodily material (HBM) for research projects aimed at better understanding and fighting this disease. 1 / Collection of blood and respiratory HBM from 500 patients with COVID-19 virus infection, as well as of post-mortem kidney and lung biopsies. 2 / Collection of blood HBM from 4000 healthy hospital and university staff members, currently not infected with the SARS-CoV2 virus, but possibly previously infected. SARS-CoV2 serology will be measured at 3 time points during the pandemia. "
Funding: Fondation Léon Frédéricq Wallonia Region ULiège CHU Liège
Contact: Pascale Huynen, Dpt of Microbiology, CHU of Liège. Stéphanie Gofflot, BHUL (CHU of Liège biobank).